Division II A Alignment
TSSAA Division II A Regions:
| East/Middle | West |
| Davidson Academy | Bishop Byrne High School |
| *Donelson Christian Academy | Evangelical Christian School |
| *Ezell-Harding | Elliston Baptist Academy |
| *Franklin Road Academy | First Assembly Christian School |
| Kings Academy | *Fayette Academy |
| Knoxville Webb | Harding Academy |
| *Lancaster Christian | Immaculate Conception School |
| Mt. Juliet Christian | *Jackson Christian School |
| *Riverside Christian | Lausanne Collegiate School |
| St. Andrews | Memphis Catholic |
| St. Cecilia | Rossville Christian Academy |
| University School of Nashville | Southern Baptist Educational Center |
| Webb School | St. George's Independent School |
| *Zion Christian | St. Mary's Episcopal School |
| Tipton-Rosemark Academy | |
| *University School of Jackson |
*Denotes schools new to Division II in 2009
Division IIA West Region:
*Denotes Football Schools
